Microplates FAQ

What are edge effects?

 A:  The “edge effect” is a discrepancy between the inner and outer wells (local environment), where each well has it’s own unique environment. Edge effects are commonly seen in high density formats as well volumes decrease as density increases.

Environmental edge effects can lead to variation across microplates, compromising the efficacy, reproducibility and reliability of cell-based assays. Fluctuations in environmental conditions, such as temperature and CO2 concentration, can trigger a variety of unwanted cellular responses and result in the evaporation of culture medium from the perimeter wells. Consequently, the micro-environment of the outer wells varies considerably from that of the inner ones, altering cell viability and assays results

What is the surface area of Vale 96 High Performance Microplate wells?

Vale 96 High Performance Microplates have a reduced surface area (half), compared to many other commercially available 96 well microplates, facilitating the use of lower reagent volumes while at the same time supporting a broad working volume of 10 – 150uL. This feature, combined with reduced evaporation due to environmental buffering, means significant savings as regards media, reagents, and valuable biological material.

Are Vale 96 High Performance Microplates compatible with imaging instruments such as confocal microscopes and high content analysers?

Vale 96 High Performance Microplates are made from imaging grade materials. They also possess flat well bottoms to facilitate imaging up to 40x magnification.

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