A: The “edge effect” is a discrepancy between the inner and outer wells (local environment), where each well has it’s own unique environment. Edge effects are commonly seen in high density formats as well volumes decrease as density increases.
Environmental edge effects can lead to variation across microplates, compromising the efficacy, reproducibility and reliability of cell-based assays. Fluctuations in environmental conditions, such as temperature and CO2 concentration, can trigger a variety of unwanted cellular responses and result in the evaporation of culture medium from the perimeter wells. Consequently, the micro-environment of the outer wells varies considerably from that of the inner ones, altering cell viability and assays results